Chaenomeles Sinensis: A Potent α-and β-Glucosidase Inhibitor

Abstract

Problem statement: Glycosidase inhibitors are vital sources for the treatment of diabetes type II with a special importance in pharmacology, food industry and biotechnology, since for diabetes control, different diets and drugs, especially herbal medicines are recommended in this era. Approach: While screening for the potent natural glycosidase inhibitors, we found the fruits of Chaenomeles sinensis (C. sinensis), as the most effective glycosidase inhibitor. The crude 80% methanolic extract of the fruits and its n-hexane, methylene chloride, ethyl acetate, n-butanol and aqueous fractions were further investigated for α-glucosidase, β-glucosidase, α-galactosidase and β-galactosidase enzyme inhibition activities. Results: All the C. sinensis extracts showed remarkable α-glucosidase and β-glucosidase inhibitory activities (at a concentration of 5 μg 210 μL reaction^-1) ranging from 82-99 and 5-85%, respectively. Among all the inhibition studies, n-butanol fraction demonstrated the highest (99%) α-glucosidase inhibitory activity, whereas minor α-galactosidase (18-35%) and β-galactosidase (10-34%) inhibitions were examined in all the fractions of C. sinensis. Conclusion: C. sinensis fruits may prove as potent natural anti-diabetic source with noteworthy α-glucosidase and β-glucosidase inhibitions, because the inhibition of these enzymes provide a strong biochemical basis for the management of type II diabetes by controlling glucose absorption. These results provide intense rationale for further animal and clinical studies.