Supercritical Fluid Extraction of Palm Carotenoids

Abstract

The extraction of carotenoids from crude palm oil was carried out in a dynamic (flow- through) supercritical fluid extraction system. The carotenoids obtained were quantified using off-line UV-visible spectrophotometry. The effects of operating pressure and temperature, flow rate of the supercritical carbon dioxide (SC-CO₂), sample size of feed used on the solubility of palm carotenoids were investigated. The results showed that the extraction of carotenoids was governed by its solubility in the SC-CO₂ and can be enhanced by increasing pressure at a constant temperature or decreasing temperature at a constant pressure. Increasing the flow rate and decreasing the sample size can reduce the extraction time but do not enhance the solubility. Palm carotenoids have very low solubility in SC-CO₂ in the range of 1.31 x 10^-4 g kg^-1 to 1.58 x 10^-3 g kg^-1 for the conditions investigated in this study. The experimental data obtained were compared with those published by other workers and correlated by a density-based equation as proposed by Chrastil.