Genetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native Fowls

Abstract

Problem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The DNA extraction was based on salting out method and used amplified polymerase chain reaction technique. Exon and intron 9-11 of the fowl leptin gene receptor was amplified to produce a 382 bp fragment. The PCR products were electrophoresed on 1% agarose gel and stained by etidium bromide. Results: Then, they were digested of amplicons with Tsp509I and revealed two alleles A and B. Data were analysed using PopGene 32 package. In this population, AA, AB, BB genotype have been identified with the 69.14, 30.16, 0.7% frequencies. A and B alleles frequencies were 0.84, 0.16, respectively. x² test didn’t signify Hardy-Weinberg equilibrium in this population (p<0.05). Conclusion: The main objectives of the current strategy in commercial broiler breeding programs aim to increased growth rate, increased breast muscle yield, decreased abdominal fat pad content, increased feed efficiency and increased overall fitness. The obtained results in the present study indicated that the A allele frequency for leptin gene receptor loci was the dominant allele.